LRAR MARRAKECH

The LRARM conducts tests for mandatory regulatory controls on the import or export of animals, or individual or flock diagnostics in the event of a pathological problem or under national programs. These biological analyses concern :

• Serological tests in animals:

  • Research for anti-Brucella antibodies in serum (FC, EAT)

  • Serum anti-chlamydia antibody search by FC, ELISA

  • FC testing for anti-coxiella antibodies in serum

  • Detection of Bluetongue virus by ELISA

  • IBR/IPV virus detection by ELISA

  • Detection of bovine leukosis virus by IDG

  • Visna maedi virus detection by IDG

  • ELISA detection of equine viral arteritis

  • CAEV detection by ELISA and IDG

• Real-time PCR diagnosis:

  • Real-time PCR diagnosis of avian infuenza type A virus

  • Diagnosis of H5 avian infuenza virus by PCR in real time

  • Real-time HP7 avian infuenza virus diagnosis via PCR

  • Real-time PCR blue tongue group virus diagnosis

• Diagnosis of animal rabies:

  • Rabies antigen detection by direct IF immunofluorescence

  • Mouse inoculation technique ( IS ).

  • Parasitological diagnosis in animals:

  • Searching for Great Fluke eggs (enrichment by sedimentation)

  • Mac-Master Method Egg Counting

  • Baerman Technique Larval Counting

  • Searching for Giemsa Staining Hemopause

  • May Grunwald Giemsa Stain Hemopause Search

  • Scabies and other ectoparasites

  • Diagnosis of culicoides

  • Varroasis

  • The Nosemose

  • Veterinary bacteriological diagnosis:

  • Search for Escherichia coli

  • Search for salmonella in birds

  • Search for salmonella in mammals

  • Search for Staphylococcus aureus

  • Streptococcus search

  • Research for Brucella spp other than Brucella ovis and Brucella canis

  • Research for Brucella ovis

  • Search for Pseudomonas

  • Corynebacterium Research

  • Pasteurella research

  • Search for Mycobacterium spp

  • Bacillus anthracis research

  • Search for Clostridium perfringens

  • Other bacillus

  • Other anaerobic germs

  • Search for enterobacteria

The laboratory carries out chemical and microbiological analyses of animal and animal products as well as vegetable preserves to ensure the hygienic quality of products throughout the food production and distribution chain. These analyses are carried out either in a formal framework or in the framework of self-monitoring. The laboratory also conducts accreditation audit visits for laboratories installed at the level of industrial units.

a. Food Microbiology Section

Food microbiology analyses are carried out on various product lines, namely milk and its derivatives, fish preserves, meat products, vegetable preserves, water, etc.

• Total Aerobic Mesophilic Flora count at 30°C

• Total Aerobic Mesophilic Flora count at 22°C

• Total Aerobic Mesophilic Flora count at 37°C

• Total Coliform counts at 30°C

• Coliform counts at 30°C (Waters)

• Coliform counts at 44°C (Waters)

• Total Aerobic Mesophilic Flora Count at 37°C (Waters)

• Search for Salmonella

• Aerogenosa pseudomonas count (Waters) • Fecal coliform count at 44°C

• Anaerobic sulfito-reducer counts

• Staphylococcus aureus counts

• Fecal Streptococcus counts at 37°C

• Enterobacteriaceae counts

• Enumeration of Escherichia.coli

• Yeast and mould counts

• Sterility control of canned animal products

• Checking the stability of canned animal products

• Count of aerogenosa pseudomonas

• Count of Bacillus auréus

• Horizontal method for Listeria monocytogenes research and enumeration: research method

• Horizontal method for Listeria monocytogenes research and enumeration: enumeration method

• Preparation of samples, stock suspension and decimal dilutions for microbiological examination

• Preparation of samples, stock suspension and decimal dilutions for microbiological examination of the peach

• Bacteriological criteria for natural mineral waters

• Human feed water:

• Total coliform and fecal coliform count method

• Human feed water:

• Optional Aerobic and Aerobic Germ Count Method

• Food water quality

• Water quality research and enumeration of intestinal enterococci

• Uses of alternative methods in microbiological analyses:

• Petrifilms in germ counts;

• IBISA and ALOA (new techniques used in Salmonella and Listeria research);

• Tempo: new techniques used in germ counts;

• Vidas: Salmonella search.

b. Food Chemistry Section

Analyses in food chemistry concern:

•  Chemical analyses of compound foods (bromatology: dry matter, protein, fat, minerals (phosphorus, calcium, etc.) ), water content, insoluble ash, cellulose, HCL insoluble ash.

• Chemical analyses of milk and derivatives (density, fat, freezing point, dry matter, alkaline phosphatase chlorides, acidity, PH, etc.)

• Determination of Total Sulphur Dioxide in Meat Products and Crustaceans (Bisulfite Assay)

• Determination of ABVT in fishery products (by steam distillation)

• Oil acidity, Saponification and peroxide index

• Aviculture and industrial water chemistry (oxidizability, hardness, chlorides, pH, residual chlorine)

• NaCl Rate

• Chlorine measurement

• Chloride analysis (feed, fishmeal, canned fish, salt, olives…)

• Determination of extract with water in tea

• Determination of net weight and drained weight, organoleptic characters

• Look for bile pigments (jaundice)

• Degree of bleeding

• Methods adapted to Honey:

• AOAC Official Method 980.23: Hydroxymethyl furfural (HMF), Spectrophotometric Method (HMF mg/kg)

• pH measurement

• Measurement of free acidity

• Humidity % measured by refractometry at 20°C

• Qualitative analysis of honey: dextrin/ fiehe reaction

c. Biochemistry/Histamine :

– Determination of histamine in fishery products by two methods:

• LERCK and BELL imetric fluorine method

• HPLC method

d. Analytical chemistry :

• Analysis of heavy metals:

• Mercury in fishery products (validated method)